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KMID : 0363620200410040052
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Journal of Korean Oriental Medicine
2020 Volume.41 No. 4 p.52 ~ p.63
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Immunostimulatory effects of the Pueraria lobata flower extract via MAPK signaling in RAW264.7 cells
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Kim Ki-Tae
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Abstract
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Objectives: In this study, we investigated the effects of Pueraria lobata Ohwi flower extracts (PLFE) on macrophages and their underlying mechanism(s) of action. PLFE increased the production of NO and cytokines (IL-6 and TNF-α) in a dose-dependent manner, indicating its immunostimulatory property. Furthermore, PLFE upregulated iNOS, COX-2, and mitogen-activated protein kinase (MAPK) signaling in RAW264.7 cells. Additionally, PLFE enhanced the phosphorylation of IκBα and subsequent IκBα degradation, thereby enabling the nuclear translocation of NF-κB. Taken together, these findings demonstrate that the immunostimulatory effects of PLFE are mediated by the nuclear translocation of the p65 subunit of NF-κB and subsequent secretion of cytokines (IL-6 and TNF-α), upregulation of iNOS and COX-2, and stimulation of MAPK signaling (JNK, ERK, and p38). Thus, PLFE may be a potential immunostimulatory therapeutic.
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KEYWORD
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Pueraria lobata Ohwi flower, PLFE, immunosimulatory effect
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